The Imaging FlowCytobot (IFCB) is an in-situ automated submersible imaging flow cytometer that generates images of particles in-flow taken from the aquatic environment.
The IFCB uses a combination of flow cytometric and video technology to capture high resolution images of suspended particles. Laser- induced fluorescence and light scattering from individual particles are measured and used to trigger targeted image acquisition; the optical and image data are then transmitted to shore in real time.Collected images during continuous monitoring can be processed externally with automated image classification software. Images can be classified to the genus or even species level with demonstrated accuracy comparable to that of human experts.
IFCB generates high resolution (~3.4 pixels/micron) images of suspended particles in the size range <10 to 150 μm (such as diatoms and dinoflagellates). The instrument continuously samples at a rate of 15ml of sea water per hour. Depending on the target population the IFCB can generate on the order of 30,000 high resolution images per hour.
|DEPLOYMENT:||Typical deployment 6 m (longer possible)|
|DEPTH:||Maximum depth of 40 m|
|TEMPERATURE RATING:||Temperature 0 to 45 °C|
|IMAGE RATE:||30,000 images per hour (depending on target)|
|RESOLUTION:||High resolution (~3.4 pixels/micron)|
|POWER:||Requires 18-36 VDC external power source|
|POWER CONSUMPTION:||Consumes 35 W (continuous sampling)|
|SCHEDULE:||Automated or interactive system|
For further information, download the IFCB Data Sheet or Contact us.
A live time-series stream of millions of images from an IFCB deployed in-situ at the Martha’s Vineyard Coastal Observatory from 2006 to present can be seen at the MVCO IFCB Dashboard. New images are uploaded to the cloud-based platform every two hours. Other IFCB Dashboards contain images from missions ranging from a shipboard application on a NASA cruise in the Arctic to a deployment at HAB sensitive Salt Pond on Cape Cod.
Links to IFCB Dashboards:
- Marthas Vineyard Coastal Observatory (MVCO)
- NASA Healy Arctic cruise
- Salt Pond
- Texas Observatory for Algal Succession Time-Series (TOAST)
Visit our Library for a complete selection of IFCB documentation and other product resources.
McLane offers a hands-on course in IFCB operation. IFCB owners and prospective owners are invited to spend one to two days at our facility.