Credit: National Oceanography Centre
The Robotic Cartridge Sampling Instrument (RoCSI) is a field-proven, 6,000 m depth-rated, in situ autonomous oceanographic sampler that collects and preserves water samples into industry standard 0.22 and 0.45 micron Sterivex™ filter cartridges for later eDNA analysis.
A 2023 technology transfer from the National Oceanography Centre (NOC), RoCSI provides high sample count capability in a compact instrument. Water samples are collected and then biologically preserved with a user-specified fixative. Fifty or more samples are possible depending on power supply and sample storage space. Optional in-line bleach flushing cleans the sample intake, reducing cross contamination. RoCSI combines a compact size with high depth rating (depth-rated to 6,000 m). Adaptable to vehicle integration, long-term mooring systems, buoy mounts, bottom landers, and shipboard applications.
Specifications
| DIMENSIONS: | 17 cm x 18 cm x 53 cm (6.7 in x 7.08 in x 20.86 in) |
| WEIGHT IN AIR: | 15.5 kg (34 lbs) |
| WEIGHT IN SEAWATER: | 10 kg (22 lbs) |
| SAMPLE COLLECTION: | Collects samples into industry standard 0.22 and 0.45 micron Sterivex™ filter cartridges. |
| DEPTH: | 6000 m |
| BULKHEAD CONNECTOR: | Subconn MCBH8M 8-pin male for 12V power. |
| COMMUNICATIONS: | USB to a Windows® interface or RS-232 for adaptive sampling. |
| POWER SUPPLY: | 12 V DC /2 A nominal; 25 V max 0.4-0.7 A current at 12 V |
RoCSI Consumables
| Filter Cartridges |
Sterivex™ GP – 0.22um PES membrane |
|
Sterivex™ HV – 0.45um PVDF membrane |
|
| Needle Free Valves |
Qosina Needleless Injection Site, Swabbable, Luer Lock https://www.qosina.com/needleless-injection-site-male-luer-lock-swabbable-80147 |
| Reagent Bags |
Sartorius Flexboy® 2D Bag for sampling – Luer Lock male – Luer Lock female |
Deployments
| 2023 (March) | RRS James Cook JC241– RoCSI integrated onto Remotely Operated Vehicle ISIS to collect eDNA from the Clarion Clipperton Zone |
| 2022 | iAtlantic JC237, RRS James Cook |
| 2020 | iMirabilis2 expedition, EU Horizon 2020 (3500 m depth) |
| 2020 | Surface RoCSI measures DNA from microbes across the western N Atlantic (Bermuda and along the US coast) – ISME Journal article |
For further information download the RoCSI Datasheet or Contact us.
Note: McLane utilizes various metals and engineering plastics in the fabrication of our instruments. These materials are usually suitable for long-term oceanographic deployments. However, it is the customer’s responsibility to determine material suitability for a specific operating environment. Review McLane statement on materials used in frames, fixtures, and housings
Read the ON & T Magazine feature article about the RoCSI technology project.